Go to https://console.akash.network/, click on “Connect Wallet.”
High performance computation is required to process next generation sequencing data and translate experimental findings from biological experiments to actionable insights. There are many benefits to using decentralized physical infrastructure networks (DePINs) for this purpose. Unlike cloud service providers that requires operational costs associated with running large scale data centers and have to pay increasingly expensive insurance premiums to insure against the weather volatility associated with climate change, DePINs hosted by smaller scale providers can offer globally competitivei prices. Here I present a technical protocol that enables permissionless, borderless processing of biological data for clinical research. This technical documentation is a demonstration of a basic Nextflow workflow using Storj and Akash Network for decentralized data storage and computing services.
nf-core is a open source software community offering many genomics pipelines developed by the global scientific community. As a demo use case, I will use nf-core/differentialabundance to perform a differential gene analysis using only DePINs. I choose this pipeline because it has low hardware requirements to run and is sufficient to demonstration that if my protocol works, then any other genomics pipeline developed by the nf-core community should work too.
The aim of a differential gene analysis is to identify genes with difference in expression patterns under different experimental conditions (gene(s) versus condition(s) and vice versa) for a set of samples. This is a key step to connect RNA sequence data with experimental conditions and translate the biological experiment into findings that can help us map changes to pathways, processes, and regulatory mechanisms to generate hypotheses about the underlying biological mechanisms. Many clinical trials and clinical experiments using non-human model organisms depend on processing RNA data to understand the genetic mechanisms underlying how treatments affect individuals.
Token Addresses:
Browser Wallet: Kepler [3]
Hardware Requirements:
System Requirements: Ubuntu 24.4
Dataset: https://link.storjshare.io/s/jwwea5lbiwqnnetf3uqbk2ptp7uq/test
Go to https://console.akash.network/, click on “Connect Wallet.”